Pma1 Antibody (40B7)

Novus Biologicals | Catalog # NB120-4645

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Yeast

Cited:

Yeast

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 40B7
View all Pma1 Primary Antibodies »

Product Specifications

Immunogen

Generated from a sequence of yeast Pma1 [Swiss-Prot# P05030]

Reactivity Notes

Saccharomyces cerevisiae.

Localization

Plasma Membrane

Marker

Plasma Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Pma1 Antibody (40B7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:10000
Application Notes
This Pma1 Antibody (40B7) is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. For Western blots of yeast protein samples (cell lysates), use at a dilution of 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL western detection methods, use at a dilution of 1:1,000 to 1:5,000. The predicted molecular weight by western blot is 100 kDa.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Supplied as concentrated hybridoma cell culture media.

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pma1

Pma1 (plasma membrane H+-ATPase) belongs to cation transport ATPase (P-type) (TC 3.A.3) family and is an essential protein in yeast/Saccharomyces cerevisiae wherein it pumps out H+ for controlling intracellular pH as well as for generating an electrochemical gradient required to drive cellular import of metabolites (this activity utilizes cellular ATP). Glucose acts as an inducer of Pma1 expression and its catalytic activity through phosphorylation at Ser-899, Thr-912 and Ser-911 sites. Upon glucose starvation, Pma1 is negatively regulated by casein kinase I (Yck1p and Yck2p) phosphorylation and glucose mediated Pma1 activation requires glucose sensor Snf3p, G-protein Gpa2p and transport/phosphorylation of glucose. Hsp30 negatively regulate Pma1 activity during heat shock conditions and contributes towards thermo-tolerance by lowering cellular ATP consumption. Pma1 expression has been shown to induce oncogenic transformation of NIH 3T3 cells and cause increased intracellular pH. Pma1 has emerged as a potential target for development of anti-fungal agents, its suitability as a biomarker for toxicity mediated stress and as a model for study of membrane protein biogenesis.

Alternate Names

Plasma membrane ATPase 1, PMA1, Proton pump 1, YGL008C

Gene Symbol

Pma1

Additional Pma1 Products

Product Documents for Pma1 Antibody (40B7)

Certificate of Analysis

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Product Specific Notices for Pma1 Antibody (40B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pma1 Antibody (40B7)

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Protocols

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