PMCA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87261

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PMCA1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PMCA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.
Western Blot: PMCA1 Antibody [NBP1-87261]

Western Blot: PMCA1 Antibody [NBP1-87261]

Western Blot: PMCA1 Antibody [NBP1-87261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human Cerebral Cortex tissue.
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody [NBP1-87261]

Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody [NBP1-87261]

Immunocytochemistry/Immunofluorescence: PMCA1 Antibody [NBP1-87261] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus & plasma membrane.
Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261]

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human kidney shows low expression as expected.

Applications for PMCA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMCA1

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Alternate Names

ATPase, Ca++ transporting, plasma membrane 1, EC 3.6.3, EC 3.6.3.8, plasma membrane calcium ATPase, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium pump, Plasma membrane calcium pump isoform 1, plasma membrane calcium-ATPase, PMCA1kb, PMCA1plasma membrane calcium-transporting ATPase 1

Gene Symbol

ATP2B1

Additional PMCA1 Products

Product Documents for PMCA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PMCA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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