PMCA4 Antibody (2G8) - Azide and BSA Free

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07]

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Western Blot: PMCA4 Antibody (2G8) [H00000493-M07]

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Sandwich ELISA: PMCA4 Antibody (2G8) [H00000493-M07] - Detection limit for recombinant GST tagged ATP2B4 is 1 ng/ml as a capture antibody.

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] -

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE & visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b & renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b & renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (2G8) - Azide and BSA Free [H00000493-M07] -

Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE and visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.