PNMT Antibody (1C11) - BSA Free

Novus Biologicals | Catalog # NBP1-97942

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C11

Format

BSA Free
View all PNMT Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PNMT (1-282aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PNMT Antibody (1C11) - BSA Free

Western Blot: PNMT Antibody (1C11)BSA Free [NBP1-97942]

Western Blot: PNMT Antibody (1C11)BSA Free [NBP1-97942]

Western Blot: PNMT Antibody (1C11) [NBP1-97942] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNMT antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (1C11) - BSA Free [NBP1-97942]

Immunocytochemistry/ Immunofluorescence: PNMT Antibody (1C11) - BSA Free [NBP1-97942]

Immunocytochemistry/Immunofluorescence: PNMT Antibody (1C11) [NBP1-97942] - Analysis in Hep3B cells. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western Blot: PNMT Antibody (1C11)BSA Free [NBP1-97942]

Western Blot: PNMT Antibody (1C11)BSA Free [NBP1-97942]

Western Blot: PNMT Antibody (1C11) [NBP1-97942] - Lane 1.: 293T cell lysate Lane 2.: PNMT transfected 293T cell lysate

Applications for PNMT Antibody (1C11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNMT

Phenylethanolamine N-methyltransferase (PNMT) catalyzes the synthesis of epinephrine and plays an important role in regulating cardiovascular function. Expression of PNMT is regulated by hormonal and neural stimuli because its promoter contains sequences responding to cholinergic and depolarization stimuli. PNMT is the final enzyme in the catecholamine pathway, catalyzing the conversion of norepinephrine to epinephrine using as the cosubstrate and methyl donor, S-adenosylmethionine.

Long Name

Phenylethanolamine N-Methyltransferase

Alternate Names

Noradrenaline N-methyltransferase, PENT, PNMTase

Gene Symbol

PNMT

UniProt

NP_002677 (Human)

Additional PNMT Products

Product Documents for PNMT Antibody (1C11) - BSA Free

Certificate of Analysis

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Product Specific Notices for PNMT Antibody (1C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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