PNMT Antibody (OTI1D2)

Novus Biologicals | Catalog # NBP2-00688

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1D2
View all PNMT Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PNMT(NP_002677) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PNMT Antibody (OTI1D2)

Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688]

Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688]

Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688] - Analysis of extracts (10ug) from 9 Human tissue by using anti-PNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunocytochemistry/ Immunofluorescence: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunocytochemistry/ Immunofluorescence: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunocytochemistry/Immunofluorescence: PNMT Antibody (1D2) [NBP2-00688] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT.
Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688]

Western Blot: PNMT Antibody (OTI1D2) [NBP2-00688]

Western Blot: PNMT Antibody (1D2) [NBP2-00688] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT.
Western Blot: PNMT Antibody (1D2) [NBP2-00688] Analysis of extracts (35ug) from 9 different cell lines by using anti-PNMT monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNMT mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (OTI1D2) [NBP2-00688]

Immunohistochemistry-Paraffin: PNMT Antibody (1D2) [NBP2-00688] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PNMT mouse monoclonal antibody.

Applications for PNMT Antibody (OTI1D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PNMT

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].

Long Name

Phenylethanolamine N-Methyltransferase

Alternate Names

Noradrenaline N-methyltransferase, PENT, PNMTase

Entrez Gene IDs

5409 (Human)

Gene Symbol

PNMT

UniProt

P11086

Additional PNMT Products

Product Documents for PNMT Antibody (OTI1D2)

Certificate of Analysis

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Product Specific Notices for PNMT Antibody (OTI1D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PNMT Antibody (OTI1D2)

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Protocols

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