Podocalyxin Like Antibody (CL0284) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43980

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0284

Format

Azide and BSA Free
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O00592, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Podocalyxin Like Antibody (CL0284) - Azide and BSA Free

Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Western Blot: Podocalyxin Like Antibody [NBP3-43980]

Western Blot: Podocalyxin Like Antibody [NBP3-43980]

Lane 1: Marker [kDa]
Lane 2: Human kidney tissue lysate
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Analysis in human kidney and liver tissues using NBP3-43980 antibody. Corresponding Podocalyxin Like RNA-seq data are presented for the same tissues.
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Staining of human kidney shows strong membranous positivity in cells in glomeruli, as well as endothelial cells.
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Staining of human liver shows no positivity in hepatocytes as expected.
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Staining of human fallopian tube shows strong positivity in apical membranes in glandular cells.
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP3-43980]

Staining of human rectum shows moderate membranous positivity in endothelial cells.
Podocalyxin Like Antibody (CL0284) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NBP3-43980]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NBP3-43980]

Staining of U-251 cells using the Anti-Podocalyxin Like monoclonal antibody).

Applications for Podocalyxin Like Antibody (CL0284) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (CL0284) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody (CL0284) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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