Podoplanin Antibody (8.1.1) [DyLight 405]

Novus Biologicals | Catalog # NB600-1015V

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy, CyTOF-ready

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Golden Syrian Hamster IgG Clone # 8.1.1
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Murine thymic stromal cell lines

Reactivity Notes

Does not cross-react with human.

Localization

Membrane; Single-pass type I membrane protein.

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Golden Syrian Hamster

Isotype

IgG

Applications for Podoplanin Antibody (8.1.1) [DyLight 405]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Podoplanin

Podoplanin (PDPN, Gp38 or Ots8) belongs to family of type-1 transmembrane sialomucin-like glycoproteins and is expressed in various tissues such as keratinocytes, synovial fibroblasts, lymphatic endothelial cells and cancerous cells. Originally identified in glomerular podocytes, PDPN is now considered as one of the most reliable markers for immunodetection of lymphatic endothelium. Its expression has been documented in benign/malignant cancers such as thymoma, osteosarcoma, laryngeal squamous cell carcinoma, odontogenic tumors as well as in CIS (carcinoma in situ)/ SCC (squamous cell carcinoma) of the oral mucosa. PDPN implicates in cellular migration and/or actin cytoskeleton organization, induction of platelet aggregation and is essential to lung cell proliferation as well as alveolus formation postpartum. PDPN expression is up-regulated by pro-inflammatory factors, such as IFN-gamma, TGF-beta, TNF-alpha and IL-1beta; and its mucin-type character has been shown to be associated with its pathophysiological functions including polarized migration of lymphatic endothelial cells as well as in invasion of cancer. PDPN knockout mice show embryonic lethality with respiratory failure and defective development of lymphoid follicles, germinal centers in spleen and lymph node remnants.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (8.1.1) [DyLight 405]

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (8.1.1) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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