Podoplanin Antibody (8.1.1) - BSA Free

Novus Biologicals | Catalog # NB600-1015

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Human (Negative)

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Golden Syrian Hamster IgG Clone # 8.1.1

Format

BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Murine thymic stromal cell lines

Reactivity Notes

Does not cross-react with human.

Localization

Membrane; Single-pass type I membrane protein.

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Golden Syrian Hamster

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Podoplanin Antibody (8.1.1) - BSA Free

Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Podoplanin-Antibody-8-1-1-BSA-Free-Western-Blot-NB600-1015-img0012.jpg
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 5 ug/ml overnight at 4C and detected with an anti-Golden Syrian hamster DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin and these are cells of a malignant serosal tumor. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)
Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Western Blot: Podoplanin Antibody (8.1.1)BSA Free [NB600-1015]

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Podoplanin-Antibody-8-1-1-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1015-img0013.jpg
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.
Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015]

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver and gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)
Podoplanin Antibody (8.1.1) - BSA Free

Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] -

Podolanin expressing on Mɸs mediates interactions with platelets.Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody ( alpha -podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Mɸs and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 μm.
Podoplanin Antibody (8.1.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] -

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/17…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (8.1.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] -

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/17…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (8.1.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] -

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/17…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (8.1.1) - BSA Free

Immunofluorescent Staining of Adult stem cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Applications for Podoplanin Antibody (8.1.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:400

Immunocytochemistry/ Immunofluorescence

1-5ug/ml

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000-1:2000
Application Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NB600-1015 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

14726 (Mouse)

Gene Symbol

PDPN

UniProt

Q62011 (Mouse)

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (8.1.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Podoplanin Antibody (8.1.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podoplanin Antibody (8.1.1) - BSA Free

Customer Reviews for Podoplanin Antibody (8.1.1) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Podoplanin Antibody (8.1.1) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse mammary gland after MDA-MB-231 orthotopic transplantation
    Species: Mouse
    Verified Customer | Posted 05/17/2011
    Podoplanin Antibody (8.1.1) - BSA Free NB600-1015

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies