Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-96423

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LF3/B7/D5B27

Format

Azide and BSA Free
View all Podoplanin Primary Antibodies »

Product Specifications

Immunogen

Membrane protein fraction of isolated rat glomeruli

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-Paraffin-NB110-96423-img0008.jpg
Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NB110-96423]

Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NB110-96423]

Western Blot: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin.
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 um. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti- Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both proteins in lymphatic endothelial cells. Note: The anti-mouse Lyve-1 polyclonal antibody shows a strong cross reaction with rat Lyve-1 protein.
Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.
Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] -

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Sarcomatoid-type malignant mesothelioma observed in the MWCNT B high-dose group. A: H & E, low magnification of tumor tissue invading the diaphragm (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing several positive cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] -

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the MWCNT D low-dose group. A: H & E, low magnification of tumor tissue found on the surface of the omentum (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of the majority of cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] -

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] -

Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] -

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the amosite asbestos group. A: H & E, low magnification of tumor tissue found on the liver surface (10×). B: H & E, higher magnification (20×). C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of many cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positivity in distinct parts of the tumor. Image collected & cropped by CiteAb from the following publication (http://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

6-30 ug/ml

Immunohistochemistry-Paraffin

6-30 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

54320 (Rat)

Gene Symbol

PDPN

UniProt

Q64294 (Rat)

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Customer Reviews for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free and earn rewards!

Have you used Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can I use (NB110-96423) PDPN antibody to sort rat PDPN+ cells, and then culture the cells?

    A: As long as the antibody against PDPN has been validated in flow, it should be able to be used in cell sorting (Fluorescence activated cell sorter); provided the antibody has to organize the extracellular domain (rat a.a. 23 - 135) or your will need to permeabilize the cells for detection and then kill them. For this, please look at the immunogens for the candidate antibodies (they have to be in the a.a. 23 - 135 region).

  • Q: How do I choose the best dilutions?

    A: The dilution can be the same as in flow. We should provide the ranges of dilution for all antibodies that have been validated in flow.

  • Q: Can I use (NB110-96423) PDPN antibody to sort rat PDPN+ cells, and then culture the cells?

    A: As long as the antibody against PDPN has been validated in flow, it should be able to be used in cell sorting (Fluorescence activated cell sorter); provided the antibody has to organize the extracellular domain (rat a.a. 23 - 135) or your will need to permeabilize the cells for detection and then kill them. For this, please look at the immunogens for the candidate antibodies (they have to be in the a.a. 23 - 135 region).

  • Q: How do I choose the best dilutions?

    A: The dilution can be the same as in flow. We should provide the ranges of dilution for all antibodies that have been validated in flow.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies