Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80915
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # LF3/B7/D5B27
Format
Azide and BSA Free
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Product Specifications
Immunogen
Membrane protein fraction of isolated rat glomeruli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
![Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Western-Blot-NBP2-80915-img0001.jpg "Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]")
Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]
Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin. Image from the standard format of this antibody.![Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80915-img0004.jpg "Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle. Image from the standard format of this antibody.![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0005.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0002.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both pr![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0003.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0006.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle. Image from the standard format of this antibody.Applications for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
6-30 ug/ml
Immunohistochemistry-Paraffin
6-30 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Gene Symbol
PDPN
Additional Podoplanin Products
Product Documents for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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