Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80915
![Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Western-Blot-NBP2-80915-img0001.jpg "Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # LF3/B7/D5B27
Format
Azide and BSA Free
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Product Specifications
Immunogen
Membrane protein fraction of isolated rat glomeruli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NBP2-80915]
Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin. Image from the standard format of this antibody.![Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80915-img0004.jpg "Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunocytochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle. Image from the standard format of this antibody.![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0005.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0002.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both pr![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0003.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]](https://resources.rndsystems.com/images/products/Podoplanin-Antibody-LF3-B7-D5B27-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80915-img0006.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]")
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915]
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NBP2-80915] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle. Image from the standard format of this antibody.Applications for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
6-30 ug/ml
Immunohistochemistry-Paraffin
6-30 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Gene Symbol
PDPN
Additional Podoplanin Products
Product Documents for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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