POLR2E Antibody (OTI3B5)

Novus Biologicals | Catalog # NBP2-00482

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B5
View all POLR2E Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2E Antibody (OTI3B5)

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

POLR2E-Antibody-OTI3B5-Western-Blot-NBP2-00482-img0013.jpg
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Human tonsil.
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then analyzed by flow cytometry.
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482]

Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482]

Immunohistochemistry-Paraffin: POLR2E Antibody (OTI3B5) [NBP2-00482] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482]

Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for POLR2E Antibody (OTI3B5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2E

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Entrez Gene IDs

5434 (Human)

Gene Symbol

POLR2E

Additional POLR2E Products

Product Documents for POLR2E Antibody (OTI3B5)

Certificate of Analysis

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Product Specific Notices for POLR2E Antibody (OTI3B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POLR2E Antibody (OTI3B5)

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Protocols

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