POLR3A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83204

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR3A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: POLR3A Antibody [NBP1-83204]

Immunocytochemistry/ Immunofluorescence: POLR3A Antibody [NBP1-83204]

Immunocytochemistry/Immunofluorescence: POLR3A Antibody [NBP1-83204] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
POLR3A Antibody - BSA Free Western Blot: POLR3A Antibody - BSA Free [NBP1-83204]

Western Blot: POLR3A Antibody - BSA Free [NBP1-83204]

Analysis in human cell lines PC-3 and MCF-7 using Anti-POLR3A antibody. Corresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
POLR3A Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR3A Antibody - BSA Free [NBP1-83204]

Chromatin Immunoprecipitation-exo-Seq: POLR3A Antibody - BSA Free [NBP1-83204]

ChIP-Exo-Seq composite graph for Anti-POLR3A (NBP1-83204) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for POLR3A Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3A

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Alternate Names

DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, DNA-directed RNA polymerase III subunit RPC1, EC 2.7.7.6, hRPC155, POL3A, polymerase (RNA) III (DNA directed) polypeptide A, 155kDa, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C160, RNA polymerase III subunit RPC155-D, RPC1, RPC155RNA polymerase III subunit C1

Entrez Gene IDs

11128 (Human)

Gene Symbol

POLR3A

UniProt

O14802 (Human)

Additional POLR3A Products

Product Documents for POLR3A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POLR3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POLR3A Antibody - BSA Free

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Protocols

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FAQs

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