POLR3F Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76929

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POLR3F Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F. The immunogen is located within the first 50 amino acids of POLR3F. Amino Acid Squence: MAEVKVKVQPPDADPVEIEN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3F Antibody - BSA Free

Western Blot: POLR3F AntibodyBSA Free [NBP1-76929]

Western Blot: POLR3F AntibodyBSA Free [NBP1-76929]

Western Blot: POLR3F Antibody [NBP1-76929] - Human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 ug/ml.
POLR3F Antibody - BSA Free

Immunohistochemistry: POLR3F Antibody - BSA Free [NBP1-76929] -

Immunohistochemistry: POLR3F Antibody - BSA Free [NBP1-76929] - Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 ug/mL.
POLR3F Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: POLR3F Antibody - BSA Free [NBP1-76929] -

Immunocytochemistry/ Immunofluorescence: POLR3F Antibody - BSA Free [NBP1-76929] - Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 ug/mL.

Applications for POLR3F Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3F

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Alternate Names

DNA-directed RNA polymerase III 39 kDa polypeptide, DNA-directed RNA polymerase III subunit F, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerases III 39 kDa polypeptide, polymerase (RNA) III (DNA directed) polypeptide F (39 kDa), polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa, RNA polymerase III 39 kDa subunit, RNA polymerase III C39 subunit, RNA polymerase III subunit C6, RPC39MGC13517, RPC6

Gene Symbol

POLR3F

UniProt

CAC11110 (Human)

Additional POLR3F Products

Product Documents for POLR3F Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POLR3F Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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