POLR3GL Antibody (OTI5E8)
Novus Biologicals | Catalog # NBP2-45367
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5E8
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Product Specifications
Immunogen
Full length human recombinant protein of human POLR3GL(NP_115681) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for POLR3GL Antibody (OTI5E8)
![Western Blot: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Western-Blot-NBP2-45367-img0023.jpg "Western Blot: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Western Blot: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Western Blot: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR3GL.![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0022.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0003.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0004.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0005.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0006.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0007.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0008.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0009.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0010.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human liver tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0011.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0012.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0013.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0014.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0015.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0016.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0017.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0018.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0019.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0020.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunohistochemistry-NBP2-45367-img0021.jpg "Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunohistochemistry: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunofluorescence-NBP2-45367-img0001.jpg "Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL.![Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]](https://resources.rndsystems.com/images/products/POLR3GL-Antibody-OTI5E8-Immunofluorescence-NBP2-45367-img0002.jpg "Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]")
Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367]
Immunofluorescence: POLR3GL Antibody (OTI5E8) [NBP2-45367] - Analysis of HeLa cells.Applications for POLR3GL Antibody (OTI5E8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:4000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POLR3GL
Alternate Names
alternative RNA polymerase III subunit 32, DNA-directed RNA polymerase III subunit G-like, DNA-directed RNA polymerase III subunit RPC7-like, flj32422, FLJ34890, MGC3200, polymerase (RNA) III (DNA directed) polypeptide G (32kD) like, polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like, RNA polymerase III subunit C7-like, RPC32HOM, RPC32-like protein
Gene Symbol
POLR3GL
Additional POLR3GL Products
Product Documents for POLR3GL Antibody (OTI5E8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for POLR3GL Antibody (OTI5E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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