Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-39021
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGPEFKRAI
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:35087510). Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining in human stomach and liver tissues using NBP2-39021 antibody. Corresponding GALNT7 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human kidney, liver, prostate and stomach using Anti-GALNT7 antibody NBP2-39021 (A) shows similar protein distribution across tissues to independent antibody NBP2-39054 (B).Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4Immunocytochemistry/ Immunofluorescence: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunocytochemistry/Immunofluorescence: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human cell line HaCaT shows localization to the Golgi apparatus. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human kidney shows very weak positivity in cells in tubules as expected.Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human liver shows no positivity in hepatocytes as expected.Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human prostate shows strong positivity in glandular cells of Golgi apparatus.Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021]
Immunohistochemistry-Paraffin: Polypeptide GalNac Transferase 7/GALNT7 Antibody [NBP2-39021] - Staining of human stomach shows strong positivity in Golgi apparatus in glandular cells.Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free [NBP2-39021] -
GALNT7 protein expression, but not mRNA expression, is altered in CD4+ T cells following activation. (A) Model of GALNT1 and GALNT7 regulation by lncSnhg7. (B, C) CD4+CD25- T conventional cells were isolated from total splenocytes. Cells were cultured in the presence of anti-CD3/CD28 magnetic beads for 0 and 16 hours for RNA or 0 and 72 hours for protein. GALNT1 and GALNT7 expression was analyzed by qPCR (B) and western blot (C). Statistical significance was assessed using ratio paired t-tests between stimulated and unstimulated samples with correction for multiple comparisons using the Holm-Šidak method in post-hoc analysis. *p < 0.05. n = 3-7 per group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35087510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free [NBP2-39021] -
miR-34a regulates GALNT7 expression in primary T Cells. (A) Optimization of miR-34a used in nucleofection. miR-34a was overexpressed in mouse CD4+ T cells (B) or EL4 cells (C). miR-34a mRNA levels were assessed using qPCR. GALNT7 levels were measured using western blotting. Data from representative experiments are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35087510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free [NBP2-39021] -
miR-34a regulates GALNT7 expression in primary T Cells. (A) Optimization of miR-34a used in nucleofection. miR-34a was overexpressed in mouse CD4+ T cells (B) or EL4 cells (C). miR-34a mRNA levels were assessed using qPCR. GALNT7 levels were measured using western blotting. Data from representative experiments are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35087510), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Polypeptide GalNAc Transferase 7/GALNT7
Long Name
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7
Alternate Names
GALNAC-T7, GalNAcT7, pp-GaNTase 7
Gene Symbol
GALNT7
UniProt
Additional Polypeptide GalNAc Transferase 7/GALNT7 Products
Product Documents for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Polypeptide GalNac Transferase 7/GALNT7 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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