Porcine IL-12 Biotinylated Antibody

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Porcine IL-12 Biotinylated Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine IL-12 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IL-12 and recombinant mouse IL-12 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer
Ile23-Asn324, Arg26-Ser222
Accession # Q28938, Q29053, respectively

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Porcine IL‑12 (Catalog # 912-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 aa addition in the p35 subunit and a 4 aa deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyer's patches, spleen and thymus.

References

Foss, D. et al. (1997) Vet. Immunol. Immunopathol. 57:121.

Long Name

Interleukin 12

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Alternate Names

CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

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SDS

Related Research Areas

Citation for Porcine IL-12 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
J. Virol., 2006;80(1):372-82.
Species: Porcine
Sample Types: Whole Cells
Applications: ELISpot Development