Porcine IL-12 Antibody Summary
Ile23-Asn324 (p40), Arg26-Ser222 (p35)
Accession # Q28938 (p40), Q29053 (p35)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑12 and Neutralization by Porcine IL‑12 Antibody. Recombinant Porcine IL‑12 (Catalog # 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND50 is typically 0.01-0.04 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.
Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 amino acid addition in the p35 subunit and a 4 amino acid deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyer's patches, spleen, and thymus.
Citation for Porcine IL-12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
J. Virol., 2006;80(1):372-82.
Sample Types: Whole Cells
Applications: ELISpot Development
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Porcine IL-12 Antibody
There are currently no reviews for this product. Be the first to review Porcine IL-12 Antibody and earn rewards!
Have you used Porcine IL-12 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image