Intracellular Staining by Flow Cytometry
|Detection of IL‑2 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells stimulated with LPS were stained with Mouse Anti-Porcine IL‑2 APC‑conjugated Monoclonal Antibody (Catalog # IC6521A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.|
IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils, endothelial cells, and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Mature porcine IL-2 shares 71% and 51% amino acid sequence identity with human and mouse IL-2, respectively.