Porcine IL-2 APC-conjugated Antibody

  
  • Species Reactivity
    Porcine
  • Specificity
    Detects porcine IL-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human, mouse, rat, bovine, canine, equine, feline, or cotton rat IL-2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 100312
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant porcine IL-2
    Ala21-Thr154
    Accession # P26891
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑2 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells stimulated with LPS were stained with Mouse Anti-Porcine IL‑2 APC‑conjugated Monoclonal Antibody (Catalog # IC6521A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils, endothelial cells, and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Mature porcine IL-2 shares 71% and 51% amino acid sequence identity with human and mouse IL-2, respectively.

  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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