POU3F3 Antibody (CL15308) - BSA Free
Novus Biologicals | Catalog # NBP3-43660
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL15308
Format
BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P20264, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for POU3F3 Antibody (CL15308) - BSA Free
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545893.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545875.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human cerebellum shows strong nuclear positivity in granular layer and molecular layer cells.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454948.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human rectum shows no positivity in glandular cells as expected.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545898.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of mouse embryo, E14 shows strong nuclear positivity in neurons in developing cerebral cortex.![POU3F3 Antibody (CL15308) [Unconjugated] Western blot: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454958.jpg "Western blot: POU3F3 Antibody (CL15308) [NBP3-43660]")
Western blot: POU3F3 Antibody (CL15308) [NBP3-43660]
Analysis in human cell line HEK 293.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545821.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human kidney shows strong nuclear positivity in cells in tubules.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454993.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-2412025154580.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human cerebral cortex shows strong nuclear positivity in neurons and glial cells.![POU3F3 Antibody (CL15308) [Unconjugated] Immunocytochemistry/ Immunofluorescence : POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.rndsystems.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251553258.jpg "Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining in SH-SY5Y cell line with POU3F3 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).Applications for POU3F3 Antibody (CL15308) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000-1:2500
Immunohistochemistry-Paraffin
1:1000-1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POU3F3
Alternate Names
Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3
Gene Symbol
POU3F3
Additional POU3F3 Products
Product Documents for POU3F3 Antibody (CL15308) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for POU3F3 Antibody (CL15308) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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