PP2A alpha [p Tyr307] Antibody (SR1588) - BSA Free

Novus Biologicals | Catalog # NBP3-21512

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1588 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from human phospho-PP2A alpha [Tyr307] (Uniprot #: P67775)

Modification

p Tyr 307

Specificity

Detects endogenous levels of total phospho-PP2A alpha [Tyr307]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PP2A alpha [p Tyr307] Antibody (SR1588) - BSA Free

Western Blot: PP2A alpha [p Tyr 307] Antibody (SR1588) [NBP3-21512] -

Western Blot: PP2A alpha [p Tyr 307] Antibody (SR1588) [NBP3-21512] - Western blot analysis using NBP3-21512 in (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF.

Applications for PP2A alpha [p Tyr307] Antibody (SR1588) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2A

Protein phosphatase type 2A (PP2A ) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways. PP2A is comprised of a core enzyme (which is made up of catalytic C and regulatory A (PR65) subunits) and a secondary regulatory B subunit. PP2A alpha refers to the alpha isoform of the PP2A catalytic subunit.

Alternate Names

PP2CA, PPP2CA, RP-C

Gene Symbol

PPP2CA

Additional PP2A Products

Product Documents for PP2A alpha [p Tyr307] Antibody (SR1588) - BSA Free

Certificate of Analysis

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Product Specific Notices for PP2A alpha [p Tyr307] Antibody (SR1588) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail