PP2C alpha/PPM1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32751

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PP2C alpha/PPM1A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PP2C alpha/PPM1A Antibody - BSA Free (NBP1-32751) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PP2C alpha/PPM1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PP2C alpha/PPM1A Antibody - BSA Free

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751]

Immunocytochemistry/ Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751]

Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPM1A protein stained by PPM1A antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751]

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751]

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP1-32751] - Human colon carcinoma, using PPM1A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000
Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751]

Western Blot: PP2C alpha/PPM1A Antibody [NBP1-32751] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000

Applications for PP2C alpha/PPM1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PP2C alpha/PPM1A

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Long Name

Protein Phosphatase 2C alpha

Alternate Names

MMPa-2, MPPa-1, Pp2c1, PPM1A

Gene Symbol

PPM1A

Additional PP2C alpha/PPM1A Products

Product Documents for PP2C alpha/PPM1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PP2C alpha/PPM1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for PP2C alpha/PPM1A Antibody - BSA Free

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Protocols

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FAQs

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