PP2C gamma/PPM1G Antibody (k1G6) - BSA Free
Novus Biologicals | Catalog # NBP1-04337
![Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]](https://resources.rndsystems.com/images/products/PP2C-gamma-PPM1G-Antibody-k1G6-Western-Blot-NBP1-04337-img0005.jpg "Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # k1G6
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human PP2C gamma/PPM1G (317-546aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free
Western Blot: PP2C gamma/PPM1G Antibody (k1G6)BSA Free [NBP1-04337]
Western Blot: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]](https://resources.rndsystems.com/images/products/PP2C-gamma-PPM1G-Antibody-k1G6-Immunocytochemistry-Immunofluorescence-NBP1-04337-img0006.jpg "Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]")
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]
Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Analysis in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1G antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]](https://resources.rndsystems.com/images/products/PP2C-gamma-PPM1G-Antibody-k1G6-Immunohistochemistry-Paraffin-NBP1-04337-img0004.jpg "Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]")
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) - BSA Free [NBP1-04337]
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (k1G6) [NBP1-04337] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Applications for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP2C gamma
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Gene Symbol
PPM1G
UniProt
Additional PP2C gamma Products
Product Documents for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PP2C gamma/PPM1G Antibody (k1G6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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