PPOX Antibody (2F10) - Azide and BSA Free

Novus Biologicals | Catalog # H00005498-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F10

Format

Azide and BSA Free
View all PPOX Primary Antibodies »

Product Specifications

Immunogen

PPOX (AAH02357, 378 a.a. ~ 477 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EASGCVLSQELFQQRAQEAAATQLGLKEMPSHCLVHLHKNCIPQYTLGHWQKLESARQFLTAHRLPLTLAGASYEGVAVNDCIESGRQAAVSVLGTEPNS

Localization

Mitochondrial. Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Specificity

PPOX - protoporphyrinogen oxidase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PPOX Antibody (2F10) - Azide and BSA Free

Western Blot: PPOX Antibody (2F10) [H00005498-M01]

Western Blot: PPOX Antibody (2F10) [H00005498-M01]

Western Blot: PPOX Antibody (2F10) [H00005498-M01] - Analysis of PPOX expression in transfected 293T cell line by PPOX monoclonal antibody (M01), clone 2F10.Lane 1: PPOX transfected lysate(50.8 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PPOX Antibody (2F10) [H00005498-M01]

Immunohistochemistry-Paraffin: PPOX Antibody (2F10) [H00005498-M01]

Immunohistochemistry-Paraffin: PPOX Antibody (2F10) [H00005498-M01] - Analysis of monoclonal antibody to PPOX on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.
Western Blot: PPOX Antibody (2F10) [H00005498-M01]

Western Blot: PPOX Antibody (2F10) [H00005498-M01]

Western Blot: PPOX Antibody (2F10) [H00005498-M01] - Analysis of PPOX over-expressed 293 cell line, cotransfected with PPOX Validated Chimera RNAi ( Cat # H00005498-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPOX monoclonal antibody (M01), clone 2F10 (Cat # H00005498-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: PPOX Antibody (2F10) [H00005498-M01]

ELISA: PPOX Antibody (2F10) [H00005498-M01]

ELISA: PPOX Antibody (2F10) [H00005498-M01] - Detection limit for recombinant GST tagged PPOX is approximately 0.1ng/ml as a capture antibody.

Applications for PPOX Antibody (2F10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPOX

This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

Alternate Names

EC 1.3.3.4, MGC8485, PPOVP, protoporphyrinogen oxidase, V290M, variegate porphyria

Entrez Gene IDs

5498 (Human)

Gene Symbol

PPOX

OMIM

176 (Human)

UniProt

AAH02357 (Human)

Additional PPOX Products

Product Documents for PPOX Antibody (2F10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PPOX Antibody (2F10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPOX Antibody (2F10) - Azide and BSA Free

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Protocols

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