PPP2R1A Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-30772
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Format
BSA Free
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Product Specifications
Immunogen
Sheep were immunized with a peptide conjugated to KLH corresponding to the sequence NH2-Asp-Asp-Ser-Leu-Try-Pro-Ile-Ala-Val-Leu-Ile--Asp-Glu-COOH. This peptide antibody corresponds to the N- terminal peptide of PPP2R1A having a MW of 65kD. The sequence used is amino acids 7-19.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for PPP2R1A Antibody - BSA Free
Western Blot: PPP2R1A Antibody [NBP3-30772] -
Western Blot: PPP2R1A Antibody [NBP3-30772] - Legend: Novus Biologicals' sheep anti PP 2A/A antibody western blot of total rat brain homogenate.Applications for PPP2R1A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
: 1:500-1:1000
Western Blot
: 1-5 ug
Application Notes
Immunoprecipitation often used in combination with Ig purified anti pp2A/C (Cat# ) to immunoprecipitate heterotrimeric pp2A..
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
Phosphate buffered saline
Format
BSA Free
Preservative
0.08% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PPP2R1A
Alternate Names
Medium tumor antigen-associated 61 kDa protein, MGC786, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PP2AAALPHA, PP2A-Aalpha, PR65A, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, protein phosphatase 2, regulatory subunit A, alpha, serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform
Gene Symbol
PPP2R1A
Additional PPP2R1A Products
Product Documents for PPP2R1A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PPP2R1A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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