PPP2R3A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87233

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPP2R3A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EQRDPFAVQKDVENDGPEPSDWDRFAAEEYETLVAEESAQAQFQEGFEDYETDEPASPSEFGNKSNKILSASLPEKCGKLQSVDEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPP2R3A Antibody - BSA Free

Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233] - Analysis in human cell lines A-431 and HeLa using Anti-PPP2R3A antibody. Corresponding PPP2R3A RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PPP2R3A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP1-87233]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP1-87233]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP1-87233] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233]

Western Blot: PPP2R3A Antibody [NBP1-87233] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for PPP2R3A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPP2R3A

PPP2R3A is a gene that codes for a protein with three isoforms, with lengths of 1150, 529, and 414 amino acids and weights of approximately 130, 61, and 48 kDa respectively. PPP2R3A is thought to modulate substrate selectivity and catalytic activity, as well as facilitate in the localization of catalytic enzymes. Current studies are being done on diseases and disorders related to this gene including retinoblastoma, ataxia, immunodeficiency, malaria, neuronitis, and breast cancer. PPP2R3A has also been shown to have interactions with ATXN7L2, PPP2CA, PPP5C, CDC6, and PPP2R1A in pathways such as the signal transduction PKA signaling, CDK5, mTOR, cell cycle regulation, Wnt signaling, glycogen metabolism, and IL-6 signaling pathways.

Alternate Names

PP2A subunit B isoform R3 isoform, PP2A subunit B isoforms B72/B130, PP2A subunit B isoforms B'-PR72/PR130, protein phosphatase 2 (formerly 2A), regulatory subunit B' (PR 72), alphaisoform and (PR 130), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit B', alpha, protein phosphatase 2, regulatory subunit B', alpha, serine/threonine-protein phosphatase 2A regulatory subunit B' subunit alpha, subunit B, R3 isoform

Gene Symbol

PPP2R3A

Additional PPP2R3A Products

Product Documents for PPP2R3A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPP2R3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPP2R3A Antibody - BSA Free

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Protocols

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FAQs

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