PPP2R3A Antibody

Novus Biologicals | Catalog # NBP2-19908

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PPP2R3A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PPP2R3A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP2R3A Antibody

Western Blot: PPP2R3A Antibody [NBP2-19908]

Western Blot: PPP2R3A Antibody [NBP2-19908]

Western Blot: PPP2R3A Antibody [NBP2-19908] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP2-19908]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP2-19908]

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP2-19908] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
PPP2R3A Antibody

Western Blot: PPP2R3A Antibody [NBP2-19908] -

Western Blot: PPP2R3A Antibody [NBP2-19908] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (NBP2-19908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PPP2R3A Antibody

Western Blot: PPP2R3A Antibody [NBP2-19908] -

Western Blot: PPP2R3A Antibody [NBP2-19908] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (NBP2-19908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PPP2R3A Antibody

Immunocytochemistry/ Immunofluorescence: PPP2R3A Antibody [NBP2-19908] -

PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term (NBP2-19908) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for PPP2R3A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPP2R3A

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

PP2A subunit B isoform R3 isoform, PP2A subunit B isoforms B72/B130, PP2A subunit B isoforms B'-PR72/PR130, protein phosphatase 2 (formerly 2A), regulatory subunit B' (PR 72), alphaisoform and (PR 130), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit B', alpha, protein phosphatase 2, regulatory subunit B', alpha, serine/threonine-protein phosphatase 2A regulatory subunit B' subunit alpha, subunit B, R3 isoform

Gene Symbol

PPP2R3A

UniProt

Q06190 (Human)

Additional PPP2R3A Products

Product Documents for PPP2R3A Antibody

Certificate of Analysis

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Product Specific Notices for PPP2R3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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