PPP2R5D Antibody

Novus Biologicals | Catalog # NB100-846

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PPP2R5D Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRAEEFLTASQEAL corresponding to C-Terminus according to NP_006236.1, NP_851307.1, NP_851308.1.

Specificity

This antibody is expected to recognize all three reported isoforms (NP_006236.1; NP_851307.1; NP_851308.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PPP2R5D Antibody

Western Blot: PPP2R5D Antibody [NB100-846]

Western Blot: PPP2R5D Antibody [NB100-846]

Western Blot: PPP2R5D Antibody [NB100-846] - Staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody [NB100-846]

Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody [NB100-846]

Immunocytochemistry/Immunofluorescence: PPP2R5D Antibody [NB100-846] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: PPP2R5D Antibody [NB100-846]

Flow Cytometry: PPP2R5D Antibody [NB100-846]

Flow Cytometry: PPP2R5D Antibody [NB100-846] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: PPP2R5D Antibody [NB100-846]

Western Blot: PPP2R5D Antibody [NB100-846]

Western Blot: PPP2R5D Antibody [NB100-846] - Staining of A431 (A), Jurkat (B) and HepG2 (C) cell lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence.

Applications for PPP2R5D Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:20000

Western Blot

0.03 - 0.3 ug/mL

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R5D

PPP2R5D is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.

Alternate Names

MGC2134, MGC8949, PP2A B subunit isoform B56-delta, PP2A B subunit isoform B'-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform R5-delta, PP2A, B subunit, B' delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B subunit, R5 delta isoform, protein phosphatase 2, regulatory subunit B', delta, regulatory subunit B (B56), delta isoform, regulatory subunit B', delta isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, deltaisoform, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform

Entrez Gene IDs

5528 (Human)

Gene Symbol

PPP2R5D

UniProt

NP_006236. (Human)

Additional PPP2R5D Products

Product Documents for PPP2R5D Antibody

Certificate of Analysis

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Product Specific Notices for PPP2R5D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPP2R5D Antibody

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Protocols

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FAQs

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