PRDM4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19926

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Monkey (100%), Rabbit (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRDM4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PRDM4. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRDM4 Antibody - BSA Free

PRDM4 Antibody

Western Blot: PRDM4 Antibody [NBP2-19926] -

Western Blot: PRDM4 Antibody [NBP2-19926] - PC-3 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRDM4 antibody (NBP2-19926) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926]

Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926]

Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926] - Mouse muscle, using PRDM4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926]

Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926]

Immunohistochemistry-Paraffin: PRDM4 Antibody [NBP2-19926] - HBL435 xenograft, using PRDM4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
PRDM4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PRDM4 Antibody [NBP2-19926] -

PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRDM4 protein stained by PRDM4 antibody (NBP2-19926) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
PRDM4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PRDM4 Antibody [NBP2-19926] -

PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRDM4 protein stained by PRDM4 antibody (NBP2-19926) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

Applications for PRDM4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRDM4

The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. An elevated expression level of this gene has been observed in PC12 cells treated with nerve growth factor, beta polypeptide (NGF). This gene is located in a chromosomal region that is thought to contain tumor suppressor genes. [provided by RefSeq]

Alternate Names

MGC45046, PFM1PR domain-containing protein 4, PR domain containing 4, PR domain zinc finger protein 4, PR-domain zinc-finger protein PFM1

Gene Symbol

PRDM4

UniProt

Q9UKN5 (Human)

Additional PRDM4 Products

Product Documents for PRDM4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PRDM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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