Primary Cells

Rat Cortical Stem Cells

Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems now offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. Every lot of R&D Systems Rat Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation (astrocytes, neurons, and oligodendrocytes). Our rigorous testing procedures ensure the highest level of quality and consistency from lot to lot. Depending on your research needs, our Rat Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.

Figure 2 A
Figure 1. R&D Systems Rat Cortical Stem Cells (Catalog # NSC001) cultured in monolayer (A) as neurosphere (B).


Quality & Consistency

  • high Nestin expression
  • multi-lineage potential

Speed & Flexibility

Irradiated Mouse Embryonic Fibroblasts

Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001)

Protocol for culturing the BG01V hES on iMEFs

Mouse embryonic fibroblasts are used to maintain and expand pluripotent human embryonic stem cells (hES) in an undifferentiated state, or to induce differentiation along somatic or germ cell lineages. R&D Systems iMEFs (Catalog # PSC001) are mitotically inactivated by irradiation rather than using pharmacological inhibitors, thus eliminating possible exposure of your ES cells to toxic agents. Each iMEF lot is confirmed negative for mycoplasma contamination using the MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B) and negative for microbial contamination.

Furthermore, each lot is tested for its ability to support undifferentiated growth of the BG01V human embryonic stem cells as assessed by Oct-4 and SSEA-4 expression.


  • Consistent
  • Ready-to-use
  • Mitotically inactivated by irradiation
  • Maintains pluripotency of human ES cells
  • Negative for mycoplasma and microbial contamination