PRKAG2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89324

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRKAG2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRKAG2 Antibody - BSA Free

Western Blot: PRKAG2 Antibody [NBP1-89324]

Western Blot: PRKAG2 Antibody [NBP1-89324]

Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody [NBP1-89324]

Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody [NBP1-89324]

Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody [NBP1-89324] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.
Western Blot: PRKAG2 Antibody [NBP1-89324]

Western Blot: PRKAG2 Antibody [NBP1-89324]

Western Blot: PRKAG2 Antibody [NBP1-89324] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324]

Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.

Applications for PRKAG2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-89324 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKAG2

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

5'-AMP-activated protein kinase subunit gamma-2

Alternate Names

AAKG, AAKG2, AMPK gamma2, CMH6, H91620p, WPWS

Gene Symbol

PRKAG2

Additional PRKAG2 Products

Product Documents for PRKAG2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PRKAG2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRKAG2 Antibody - BSA Free

Customer Reviews for PRKAG2 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used PRKAG2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • PRKAG2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cell lysate and 293 whole cell lysate
    Species: Human
    Verified Customer | Posted 10/04/2020
    biorad chemidoc
    PRKAG2 Antibody - BSA Free NBP1-89324

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies