Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 44D9

Format

BSA Free
View all Procalcitonin Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of human Procalcitonin conjugated with carrier protein.

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Procalcitonin Antibody (44D9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection): NBP1-79064 - NB120-14817.

Reviewed Applications

Read 1 review rated 5 using NB120-14817 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Procalcitonin

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Alternate Names

CALC1, CALCA, Calcitonin 1, calcitonin gene-related peptide 1, CGRP, Katacalcin

Entrez Gene IDs

796 (Human)

Gene Symbol

CALCA

UniProt

P06881 (Human)

Additional Procalcitonin Products

Product Documents for Procalcitonin Antibody (44D9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Procalcitonin Antibody (44D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Procalcitonin Antibody (44D9) - BSA Free

Customer Reviews for Procalcitonin Antibody (44D9) - BSA Free (1)

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  • Procalcitonin Antibody (44d9)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Cancer Tissue
    Species: Human
    Verified Customer | Posted 07/15/2017
    Immunohistochemical staining of procalcitonin in tumor tissue from a patient with colonic neuroendocrine carcinoma. Procalcitonin is expressed in cytoplasm of tumor cell.
    The dilution of 1:300 is applicable for immunohistochemistry.
    Procalcitonin Antibody (44D9) - BSA Free NB120-14817

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Protocols

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FAQs for Procalcitonin Antibody (44D9) - BSA Free

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  • Q: One of our customers wants to buy PCT antibodies for ELISA detection from Novus and he has searched all the PCT antibodies on Novus website and has some questions as follows: 1. NB110-16925 (On the Novus website, NBP1-79065 is recommended for coating and NB110-16925 is for Conjugate. I want to know whether you have done some validation about your suggestion to confirm this recommended pair for sandwich immunoassay is the best choice. If so, I will not try other optimization such as coating plate with NB110-16925 and using NBP1-79065 for conjugate so as not to waste time.) 2. NB120-14817 (My question is the same as NB110-16925 and items below is the recommendation from Novus website: Recommended pairs for sandwich immunoassay (coating - conjugate): NBP1-79064(Coating) - NB120-14817(Conjugate)). 3. NB110-13044 (Do you have any recommendation pairs for this antibody? There is no suggestion about sandwich immunoassay on Novus website.)

    A: To answer your questions the antibodies that are recommended as a pair are in fact ones that have been tested together and shown as effective sandwich pairs. We will of course always guarantee all stated species and applications, but the ones that have indications to be used together are the ones we recommend for the customers. In regards to the last antibody I am not sure what pair was used for that because I do not see it in the notes so I cannot say with 100% certainty which one would be best to use for that one and would try to stick to the others.

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