ProDots Recombinant Human BMP-4 Protein

Name: ProDots Recombinant Human BMP-4 Protein
Brand: R&D Systems
SKU: PRD314

R&D Systems | Catalog # PRD314

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Product Documents

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD314 has been discontinued. View all BMP-4 products.

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source

NS0

Accession Number

Q53XC5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all BMP-4 Proteins and Enzymes »

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293 - Arg408

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser293

Predicted Molecular Mass

13 kDa (monomer)

SDS-PAGE

22-25 kDa, reducing conditions
37-41, non-reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.
The ED₅₀ for this effect is 2.5-15.0 ng/mL.

Scientific Data Images for ProDots Recombinant Human BMP-4 Protein

ProDots Recombinant Human BMP-4 Protein Bioactivity

ProDots^®Recombinant Human BMP-4 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 2.5-15.0 ng/mL.

ProDots Recombinant Human BMP-4 Protein SDS-PAGE

1 μg/lane of ProDots^®Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing multiple bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in the gel are due to variable glycosylation.

Formulation, Preparation, and Storage

PRD314

Reconstitution	For a stock solution, reconstitute at 50-200 μg/mL in sterile 4 mM HCl, or simply roll ProDot^® directly into cell culture medium for immediate use.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8°C under sterile conditions after reconstitution. • 3 months at -20 to -80°C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: BMP-4

BMP-4 forms homodimers or heterodimers with BMP-2 and signals through receptor complexes composed of type I (primarily ActRIA or BMPR-IA) and type II (primarily ActRIIA or BMPR-II) receptors. BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.