ProDots Recombinant Human IL-4 Protein

Name: ProDots Recombinant Human IL-4 Protein
Brand: R&D Systems
SKU: PRD204

R&D Systems | Catalog # PRD204

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Product Documents

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD204 has been discontinued. View all IL-4 products.

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source

E. coli

Accession Number

P05112

Applications

Bioactivity

View all IL-4 Proteins and Enzymes »

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

Product Specifications

Source

E. coli-derived human IL-4 protein
His25 - Ser153, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

14kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.

The ED₅₀ for this effect is 0.05-0.2 ng/mL.

Scientific Data Images for ProDots Recombinant Human IL-4 Protein

ProDots Recombinant Human IL-4 Protein Bioactivity

ProDots^®Recombinant Human IL-4 stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is 0.05-0.2 ng/mL.

ProDots Recombinant Human IL-4 Protein SDS-PAGE

1 μg/lane of ProDots^®Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at approximately 14 kDa.

Formulation, Preparation, and Storage

PRD204

Reconstitution	For a stock solution, reconstitute at 100-200 μg/mL in PBS containing ≥ 0.1% human or bovine serum albumin, or simply roll ProDot^® directly into cell culture medium for immediate use.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -70 °C under sterile conditions after reconstitution

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naÃ¯ve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals.