Prohibitin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33463

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Chicken (94%), Porcine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Prohibitin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin 2. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (86%).

Localization

Mitochondrion inner membrane, Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prohibitin 2 Antibody - BSA Free

Western Blot: Prohibitin 2 Antibody [NBP1-33463]

Western Blot: Prohibitin 2 Antibody [NBP1-33463]

Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody [NBP1-33463]

Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody [NBP1-33463]

Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP1-33463] - Analysis of paraformaldehyde-fixed A431, using REA (NBP1-33463) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded mouse urinary bladder. Prohibitin 2 antibody diluted at 1:500.
Western Blot: Prohibitin 2 Antibody [NBP1-33463]

Western Blot: Prohibitin 2 Antibody [NBP1-33463]

Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463]

Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.

Applications for Prohibitin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prohibitin 2

REA, or repressor of estrogen receptor activity, (also known as BAP37, or B-cell receptor-associated protein) is a 37 kD erythrocyte band 7 integral membrane protein that contains an SPFH domain. It is located in the inner mitochondrial membrane that selectively potentiates the inhibitory effectiveness of antiestrogen-occupied ER. REA plays a possible role in cell aging and acts as a mitochondrial protein chaperone. This protein has been shown to interact with the estrogen receptor, prohibitin, and IgM.

Alternate Names

BAP, Bap37, BCAP37, D-Prohibitin, p22, PHB2, PNAS-141, REA

Entrez Gene IDs

11331 (Human); 12034 (Mouse)

Gene Symbol

PHB2

UniProt

Q99623

Additional Prohibitin 2 Products

Product Documents for Prohibitin 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Prohibitin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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