Prohibitin Antibody (0V2R10)

Novus Biologicals | Catalog # NBP3-15756

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0V2R10 expressed in HEK293
View all Prohibitin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin (P35232). ASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prohibitin Antibody (0V2R10)

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756]

Western Blot: Prohibitin Antibody (0V2R10) [NBP3-15756] - Western blot analysis of extracts of various cell lines, using Prohibitin antibody (NBP3-15756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded mouse kidney tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded human colon carcinoma tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded human thyroid cancer tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded rat kidney tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded human liver tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Prohibitin Antibody (0V2R10)

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] -

Immunohistochemistry: Prohibitin Antibody (0V2R10) [NBP3-15756] - Immunohistochemistry analysis of Prohibitin in paraffin-embedded rat liver tissue using Prohibitin Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for Prohibitin Antibody (0V2R10)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression.

Alternate Names

PHB

Gene Symbol

PHB1

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (0V2R10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Prohibitin Antibody (0V2R10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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