Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-08752
![Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]](https://resources.rndsystems.com/images/products/Prohibitin-Antibody-PHB-3194-Western-Blot-NBP3-08752-img0004.jpg "Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PHB/3194
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232)
Localization
Cytoplasmic (Mitochondria)
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07841).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Western Blot: Prohibitin Antibody (PHB/3194)Azide and BSA Free [NBP3-08752]
Western Blot: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).![Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.rndsystems.com/images/products/Prohibitin-Antibody-PHB-3194-Immunocytochemistry-Immunofluorescence-NBP3-08752-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); RedDot is used to label the nuclei.![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.rndsystems.com/images/products/Prohibitin-Antibody-PHB-3194-Immunohistochemistry-Paraffin-NBP3-08752-img0003.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.rndsystems.com/images/products/Prohibitin-Antibody-PHB-3194-Immunohistochemistry-Paraffin-NBP3-08752-img0002.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).![Protein Array: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]](https://resources.rndsystems.com/images/products/Prohibitin-Antibody-PHB-3194-Protein-Array-NBP3-08752-img0005.jpg "Protein Array: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]")
Protein Array: Prohibitin Antibody (PHB/3194) - Azide and BSA Free [NBP3-08752]
Protein Array: Prohibitin Antibody (PHB/3194) [NBP3-08752] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Prohibitin (PHB/3194).Applications for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Prohibitin
Alternate Names
PHB
Gene Symbol
PHB1
Additional Prohibitin Products
Product Documents for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Prohibitin Antibody (PHB/3194) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
