Prohibitin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19944

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (96%), Chimpanzee (100%), Feline (100%), Rhesus Macaque (100%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Prohibitin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Reactivity Notes

Cat (100%), Xenopus laevis (89%).

Localization

Mitochondrion inner membrane

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Prohibitin Antibody - BSA Free (NBP2-19944) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Prohibitin Antibody - BSA Free

Western Blot: Prohibitin Antibody [NBP2-19944]

Western Blot: Prohibitin Antibody [NBP2-19944]

Western Blot: Prohibitin Antibody [NBP2-19944] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.
Prohibitin Antibody - BSA Free

Western Blot: Prohibitin Antibody [NBP2-19944] -

Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (NBP2-19944) dilution: 1:1000
Prohibitin Antibody - BSA Free

Western Blot: Prohibitin Antibody [NBP2-19944] -

Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (NBP2-19944) dilution: 1:1000
Prohibitin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody [NBP2-19944] -

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: Prohibitin protein stained by Prohibitin antibody (NBP2-19944) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Prohibitin Antibody - BSA Free

Immunohistochemistry: Prohibitin Antibody [NBP2-19944] -

Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (NBP2-19944) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for Prohibitin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

Alternate Names

PHB

Gene Symbol

PHB1

UniProt

P35232

Additional Prohibitin Products

Product Documents for Prohibitin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Prohibitin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Diabetes
Obesity

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Protocols

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