Prolactin R Antibody (U5)

Novus Biologicals | Catalog # NB300-561

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine, Hamster, Rabbit, Sheep

Cited:

Human, Rat, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # U5
View all Prolactin R Primary Antibodies »

Product Specifications

Immunogen

Purified rat liver PRL receptor.

Epitope

This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 2707162). Bovine reactivity reported in scientific literature (PMID: 25212395). Mouse reactivity reported in scientific literature (PMID: 19211889). Hamster reactivity reported in scientific literature (PMID: 24142695). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Prolactin R Antibody (U5)

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561]

Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561]

Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Mammary gland tissue from lactating light horse mare (equine) was incubated in anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23C and for 24 hours at 4C. Second antibody was biotinylated goat anti-mouse IgG at 23C for 1 hour. Development using avidin-biotin complex 23C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin.
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561]

Immunocytochemistry/Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561]

Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561]

Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerhans using NB300-561.

Applications for Prolactin R Antibody (U5)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
May be useful in IHC, IP, and WB. WB: Detects an approx. 40 kDa and 100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. IHC: Staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor. Use in ICC/IF was reported in scientific literature (PMID: 29874677).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with 0.1 ml PBS.

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prolactin R

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Long Name

Prolactin Receptor

Alternate Names

PRLR, ProlactinR

Entrez Gene IDs

5618 (Human); 24684 (Rat); 414916 (Porcine); 100009046 (Rabbit)

Gene Symbol

PRLR

UniProt

P16471

Additional Prolactin R Products

Product Documents for Prolactin R Antibody (U5)

Certificate of Analysis

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Product Specific Notices for Prolactin R Antibody (U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Prolactin R Antibody (U5)

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Protocols

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