Proteasome 20S alpha 3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-97026

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Proteasome 20S alpha 3 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Proteasome 20S alpha 3 (Accession#: AAH29402.1; Met1-Met255)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Proteasome 20S alpha 3 Antibody - Azide and BSA Free

Western Blot: Proteasome 20S alpha 3 Antibody [NBP2-97026]

Western Blot: Proteasome 20S alpha 3 Antibody [NBP2-97026]

Western Blot: Proteasome 20S alpha 3 Antibody [NBP2-97026] - Anti-Proteasome 20S alpha 3 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: MOLT4 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 30 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Proteasome 20S alpha 3 Antibody [NBP2-97026]

Immunoprecipitation: Proteasome 20S alpha 3 Antibody [NBP2-97026]

Immunoprecipitation: Proteasome 20S alpha 3 Antibody [NBP2-97026] - Proteasome 20S alpha 3 was immunoprecipitated using: Lane A: 0.5 mg MOLT-4 Whole Cell Lysate. Lane B: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane C: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-Proteasome 20S alpha 3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Proteasome 20S alpha 3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa
Proteasome 20S alpha 3 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 3 Antibody [NBP2-97026] -

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 3 Antibody [NBP2-97026] - Immunofluorescence staining of Proteasome 20S alpha 3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97026 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.

Applications for Proteasome 20S alpha 3 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Proteasome subunit alpha type-3

Alternate Names

HC8, PSC8, PSMA3

Gene Symbol

PSMA3

Additional Proteasome 20S alpha 3 Products

Product Documents for Proteasome 20S alpha 3 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Proteasome 20S alpha 3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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