Proteasome 20S beta 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38629

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Proteasome 20S beta 3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human Proteasome 20S beta 3 (NP_002786.2).

Sequence:
MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome 20S beta 3 Antibody - BSA Free

Proteasome 20S beta 3 Antibody

Western Blot: Proteasome 20S beta 3 Antibody [NBP3-38629] -

Western Blot: Proteasome 20S beta 3 Antibody [NBP3-38629] - Western blot analysis of various lysates using Proteasome 20S beta 3 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Proteasome 20S beta 3 Antibody

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] -

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Proteasome 20S beta 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Proteasome 20S beta 3 Antibody

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] -

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Proteasome 20S beta 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Proteasome 20S beta 3 Antibody

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] -

Immunohistochemistry: Proteasome 20S beta 3 Antibody [NBP3-38629] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Proteasome 20S beta 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for Proteasome 20S beta 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12.

Long Name

Proteasome subunit beta type-3

Alternate Names

Proteasome chain 13, PSMB3

Gene Symbol

PSMB3

Additional Proteasome 20S beta 3 Products

Product Documents for Proteasome 20S beta 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Proteasome 20S beta 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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