Protein A Antibody

Catalog # Availability Size / Price Qty
MAB99761-100
MAB99761-SP
Detection  of Protein A by Western Blot.
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Protein A Antibody Summary

Specificity
Detects recombinant Protein A in direct ELISA and Western blots.
Source
Monoclonal Rat IgG2A Clone # 946418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant Protein A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Protein A by Western Blot. View Larger

Detection of Protein A by Western Blot. Western blot shows Recombinant Protein A Long, Recombinant Protein A, and Recombinant Protein G (negative control). The left panel of the PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 33 kDa and Protein A Long at approximately 55 kDa (as indicated). To show specificity, the right panel was probed with Anti-Rat IgG Antibody (negative control). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein A

The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is used in purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Entrez Gene IDs
3919448 (S. aureus)
Alternate Names
Immunoglobulin G binding protein A; Protein A; SPA; Staphylococcal protein A

Product Datasheets

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