Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84051

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Protein Disulfide Isomerase/P4HB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Protein Disulfide Isomerase/P4HB Recombinant Protein corresponding to amino acids: LPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNF

Marker

Fibroblast Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Protein Disulfide Isomerase/P4HB Antibody - BSA Free Western Blot: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP1-84051]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP1-84051]

Analysis in human cell line HepG2.
Protein Disulfide Isomerase/P4HB Antibody - BSA Free Western Blot: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP1-84051]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP1-84051]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

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  • Q: We purchased  Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] and are wondering if it's specific for the monomeric version of the protein or the tetrameric version? We believe this antibody is staining primary cells (isolated from mice) that are not fibroblasts. Do you know if this antibody can stain primary cells that are not fibroblasts?

    A: Our lab has not tested this Ab with the monomeric or multimeric subunits of the protein targets.  However, the immunogen for NBP1-84051 is a.a. 236 - 379 of mouse protein (Uniprot/P09103) and  since it is a polyclonal antibody (multiple epitopes), there are very strong chances that it could detect multimers also. Our lab has performed ICC/IF staining with NBP1-84051 on human U-2 OS cell line and we have not  tested murine primary cells or fibroblasts yet. However, we strongly believe thatthis antibody will detect P4HB in any tissues/organs, cell lines/primary cells and fibroblasts as long as they express this protein.

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