Protein Phosphatase 1 beta Antibody (5O9E7)

Novus Biologicals | Catalog # NBP3-16389

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O9E7 expressed in HEK293
View all Protein Phosphatase 1 beta Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 228-327 of human Protein Phosphatase 1 beta (P62140). ADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Phosphatase 1 beta Antibody (5O9E7)

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Western Blot: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Western blot analysis of extracts of various cell lines, using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunofluorescence analysis of U-2 OS cells using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389]

Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunofluorescence analysis of NIH-3T3 cells using Protein Phosphatase 1 beta Rabbit mAb (NBP3-16389) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Protein Phosphatase 1 beta Antibody (5O9E7)

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] -

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry analysis of Protein Phosphatase 1 beta in paraffin-embedded human pancreas tissue using Protein Phosphatase 1 beta Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Protein Phosphatase 1 beta Antibody (5O9E7)

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] -

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry analysis of Protein Phosphatase 1 beta in paraffin-embedded mouse colon tissue using Protein Phosphatase 1 beta Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Protein Phosphatase 1 beta Antibody (5O9E7)

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] -

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry analysis of Protein Phosphatase 1 beta in paraffin-embedded human breast cancer tissue using Protein Phosphatase 1 beta Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Protein Phosphatase 1 beta Antibody (5O9E7)

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] -

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry analysis of Protein Phosphatase 1 beta in paraffin-embedded human esophagus tissue using Protein Phosphatase 1 beta Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Protein Phosphatase 1 beta Antibody (5O9E7)

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] -

Immunohistochemistry: Protein Phosphatase 1 beta Antibody (5O9E7) [NBP3-16389] - Immunohistochemistry analysis of Protein Phosphatase 1 beta in paraffin-embedded rat colon tissue using Protein Phosphatase 1 beta Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for Protein Phosphatase 1 beta Antibody (5O9E7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Phosphatase 1 beta

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Alternate Names

EC 3.1.3.16, EC 3.1.3.53, MGC3672, PP1beta, PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1-beta, protein phosphatase 1-delta, serine/threonine protein phosphatase PP1-beta catalytic subunit, serine/threonine-protein phosphatase PP1-beta catalytic subunit

Gene Symbol

PPP1CB

Additional Protein Phosphatase 1 beta Products

Product Documents for Protein Phosphatase 1 beta Antibody (5O9E7)

Certificate of Analysis

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Product Specific Notices for Protein Phosphatase 1 beta Antibody (5O9E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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