Protein Phosphatase 1C gamma Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-03445
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human Protein Phosphatase 1C gamma (NP_002701.1). MADLDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKKPNATRPVTPPRGMITKQAKK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protein Phosphatase 1C gamma Antibody - BSA Free
Western Blot: Protein Phosphatase 1C gamma AntibodyBSA Free [NBP3-03445]
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-03445] - Analysis of extracts of various cell lines, using Protein Phosphatase 1C gamma antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Western Blot: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445]
Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-03445] - Analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using Protein Phosphatase 1C gamma antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] -
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of H9C2 cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] -
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of U2OS cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] -
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1C gamma Antibody - BSA Free [NBP3-03445] - Immunofluorescence analysis of L929 cells using [KO Validated] Protein Phosphatase 1C gamma Rabbit pAb (A4035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Protein Phosphatase 1C gamma Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:200 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Protein Phosphatase 1C gamma
Alternate Names
EC 3.1.3.16, PP-1G, PP1gamma, PPP1G, protein phosphatase 1, catalytic subunit, gamma isoform, protein phosphatase 1, catalytic subunit, gamma isozyme, Protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Gene Symbol
PPP1CC
Additional Protein Phosphatase 1C gamma Products
Product Documents for Protein Phosphatase 1C gamma Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Protein Phosphatase 1C gamma Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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