Proteome Profiler Human Protease Array Kit

Introduction

R&D Systems Proteome Profiler™ Human Protease Array Kit (Catalog # ARY021) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 34 proteases in a single sample. No specialized equipment is necessary.

• Product Datasheet
• Troubleshooting Guide
• Assays for Analytes represented in the Human Protease Array Kit

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.