Proteome Profiler Human Protease Array Kit

Newer Version Available: ARY021B
Contains 4 membranes - each spotted in duplicate with 34 different protease antibodies

Discontinued Product

ARY021 has been discontinued and is replaced by ARY021B.

R&D Systems Proteome Profiler Antibody Array Kits
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Product Details
Citations (20)

Proteome Profiler Human Protease Array Kit Summary


R&D Systems Proteome Profiler™ Human Protease Array Kit (Catalog # ARY021) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 34 proteases in a single sample. No specialized equipment is necessary.

Simultaneously detect the relative levels of these proteases in a single sample.

ADAM8 Cathepsin X/Z/P MMP-3
ADAMTS1 Kallikrein 3/PSA MMP-8
ADAMTS13 Kallikrein 5 MMP-9
Cathepsin A Kallikrein 6 MMP-12
Cathepsin B Kallikrein 7 MMP-13
Cathepsin C/DPPI Kallikrein 10 Neprilysin/CD10
Cathepsin D Kallikrein 11 Presenilin-1
Cathepsin E Kallikrein 13 Proprotein Convertase 9
Cathepsin L MMP-1 Proteinase 3
Cathepsin S MMP-2 uPA/Urokinase
Cathepsin V

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.


Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Citations for Proteome Profiler Human Protease Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics
    Authors: C Giannasi, L Mangiavini, S Niada, A Colombo, E Della Mort, V Vismara, A Ambrosanio, P Savadori, S Casati, GM Peretti, AT Brini
    Pharmaceutics, 2022;14(6):.  2022
  2. Decidual macrophage derived MMP3 contributes to extracellular matrix breakdown in spiral artery remodeling in early human pregnancy
    Authors: Y Pan, L Yang, D Chen, H Hou, M Zhang, M Chen, F Ning, Q Lu, M Zhao, L Li, GE Lash
    Journal of reproductive immunology, 2022;150(0):103494.  2022
  3. Kallikrein-Related Peptidase 6 Is Associated with the Tumour Microenvironment of Pancreatic Ductal Adenocarcinoma
    Authors: JB Candido, O Maiques, M Boxberg, V Kast, E Peerani, E Tomás-Bort, W Weichert, A Sananes, N Papo, V Magdolen, V Sanz-Moren, D Loessner
    Cancers, 2021;13(16):.  2021
  4. Pinosylvin inhibits migration and invasion of nasopharyngeal carcinoma cancer cells via regulation of epithelial?mesenchymal transition and inhibition of MMP?2
    Authors: YC Chuang, MC Hsieh, CC Lin, YS Lo, HY Ho, MJ Hsieh, JT Lin
    Oncology reports, 2021;46(1):.  2021
  5. Anti-Periodontitis Effect of Ethanol Extracts of Alpinia Katsumadai Seeds
    Authors: SW Shin, YS Hwang
    Nutrients, 2021;14(1):.  2021
  6. Gingival Crevicular Fluid Zinc- and Aspartyl-Binding Protease Profile of Individuals with Moderate/Severe Atopic Dermatitis
    Authors: F Valenzuela, J Fernández, M Aroca, C Jiménez, D Albers, M Hernández, A Fernández
    Biomolecules, 2020;10(12):.  2020
  7. S100A8 & S100A9: Alarmin mediated inflammation in tendinopathy
    Authors: LAN Crowe, M McLean, SM Kitson, EG Melchor, K Patommel, HM Cao, JH Reilly, WJ Leach, BP Rooney, SJ Spencer, M Mullen, M Chambers, GAC Murrell, IB McInnes, M Akbar, NL Millar
    Sci Rep, 2019;9(1):1463.  2019
  8. Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization
    Authors: J Lee, YH Nho, SK Yun, YS Hwang
    Integr Cancer Ther, 2019;18(0):1534735419851.  2019
  9. Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner
    Authors: X Zhang, YS Hwang
    Oncol Lett, 2019;18(5):4645-4650.  2019
  10. Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction
    Authors: K Saito, M Sakaguchi, S Maruyama, H Iioka, EW Putranto, IW Sumardika, N Tomonobu, T Kawasaki, K Homma, E Kondo
    J Cancer, 2018;9(16):2916-2929.  2018
  11. Intranasal delivery of VEGF enhances compensatory lung growth in mice
    Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
    PLoS ONE, 2018;13(6):e0198700.  2018
  12. Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3
    Authors: SA Greenall, J Donoghue, TG Johns, TE Adams
    Transl Oncol, 2018;11(4):971-978.  2018
  13. HIV Reprograms Human Airway Basal Stem/Progenitor Cells to Acquire a Tissue-Destructive Phenotype
    Authors: NPY Chung, X Ou, KMF Khan, J Salit, RJ Kaner, RG Crystal
    Cell Rep, 2017;19(6):1091-1100.  2017
  14. Inhibition of cathepsin S confers sensitivity to methyl protodioscin in oral cancer cells via activation of p38 MAPK/JNK signaling pathways
    Authors: MJ Hsieh, CW Lin, MK Chen, SY Chien, YS Lo, YC Chuang, YT Hsi, CC Lin, JC Chen, SF Yang
    Sci Rep, 2017;7(0):45039.  2017
  15. Astrocytic tight junctions control inflammatory CNS lesion pathogenesis
    Authors: S Horng, A Therattil, S Moyon, A Gordon, K Kim, AT Argaw, Y Hara, JN Mariani, S Sawai, P Flodby, ED Crandall, Z Borok, MV Sofroniew, C Chapouly, GR John
    J. Clin. Invest., 2017;0(0):.  2017
  16. Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.  2016
  17. Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
    Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
    J Immunol, 2015;195(6):2763-73.  2015
  18. Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells.
    Authors: Mohammed F, Khajah M, Yang M, Brackenbury W, Luqmani Y
    Int J Oncol, 2015;48(1):73-83.  2015
  19. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
    Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
    Placenta, 2014;35(12):1049-56.  2014
  20. Shedding of dipeptidyl peptidase 4 is mediated by metalloproteases and up-regulated by hypoxia in human adipocytes and smooth muscle cells.
    Authors: Rohrborn D, Eckel J, Sell H
    FEBS Lett, 0;588(21):3870-7.  0


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