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Proteome Profiler Human Protease Array Kit

Contains 4 membranes - each spotted in duplicate with 35 different protease antibodies
  (9 citations)     
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Citations (9)
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Kit Summary

To simultaneously detect the relative changes of 35 proteases in a single sample. No specialized equipment is necessary

 

Troubleshooting Guide
 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.

Stability and Storage

Store the unopened product at 2° C to 8° C. Do not use past expiration date.

 

Simultaneously detect the relative levels of these proteases in a single sample.
ADAM8 Cathepsin X/Z/P MMP-7
ADAM9 DPPIV/CD26 MMP-8
ADAMTS1 Kallikrein 3/PSA MMP-9
ADAMTS13 Kallikrein 5 MMP-10
Cathepsin A Kallikrein 6 MMP-12
Cathepsin B Kallikrein 7 MMP-13
Cathepsin C/DPPI Kallikrein 10 Neprilysin/CD10
Cathepsin D Kallikrein 11 Presenilin-1
Cathepsin E Kallikrein 13 Proprotein Convertase 9
Cathepsin L MMP-1 Proteinase 3
Cathepsin S MMP-2 uPA/Urokinase
Cathepsin V MMP-3  

Assays for Analytes represented in the Human Protease Array Kit

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
ADAM8   DY1031
ADAM9   DY939
ADAMTS1   DY2197
ADAMTS13 DADT130  
Cathepsin A    
Cathepsin B DCATB0 DY953, DY2176
Cathepsin C/DPPI    
Cathepsin D    
Cathepsin E    
Cathepsin L   DY952
Cathepsin S   DY1183, DY2227
Cathepsin V   DY1080
Cathepsin X/Z/P    
DPPIV/CD26   DY1180
Kallikrein 3/PSA DKK300 DY1344
Kallikrein 5 DKK500  
Kallikrein 6    
Kallikrein 7    
Kallikrein 10    
Kallikrein 11    
Kallikrein 13    
MMP-1 DMP100  
MMP-2 MMP200 DY902
MMP-3 DMP300 DY513
MMP-7 DMP700 DY907
MMP-8 DMP800 DY908
MMP-9 DMP900 DY911
MMP-10 DM1000 DY910
MMP-12    
MMP-13 DM1300 DY511, DY913
Neprilysin   DY1182
Presenilin-1   DY149
Proprotein Convertase 9 DPC900 DY3888
Proteinase 3   DY6134-05
uPA/Urokinase DUPA00 DY1310

Product Datasheets
Product Datasheet

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

SDS
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
 Sample Type
  1. Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3
    Authors: SA Greenall, J Donoghue, TG Johns, TE Adams
    Transl Oncol, 2018;11(4):971-978.  2018
  2. Intranasal delivery of VEGF enhances compensatory lung growth in mice
    Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
    PLoS ONE, 2018;13(6):e0198700.  2018
  3. Inhibition of cathepsin S confers sensitivity to methyl protodioscin in oral cancer cells via activation of p38 MAPK/JNK signaling pathways
    Authors: MJ Hsieh, CW Lin, MK Chen, SY Chien, YS Lo, YC Chuang, YT Hsi, CC Lin, JC Chen, SF Yang
    Sci Rep, 2017;7(0):45039.  2017
  4. HIV Reprograms Human Airway Basal Stem/Progenitor Cells to Acquire a Tissue-Destructive Phenotype
    Authors: NPY Chung, X Ou, KMF Khan, J Salit, RJ Kaner, RG Crystal
    Cell Rep, 2017;19(6):1091-1100.  2017
  5. Astrocytic tight junctions control inflammatory CNS lesion pathogenesis
    Authors: S Horng, A Therattil, S Moyon, A Gordon, K Kim, AT Argaw, Y Hara, JN Mariani, S Sawai, P Flodby, ED Crandall, Z Borok, MV Sofroniew, C Chapouly, GR John
    J. Clin. Invest., 2017;0(0):.  2017
  6. Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.  2016
  7. Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells.
    Authors: Mohammed F, Khajah M, Yang M, Brackenbury W, Luqmani Y
    Int J Oncol, 2015;48(1):73-83.  2015
  8. Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
    Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
    J Immunol, 2015;195(6):2763-73.  2015
  9. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
    Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
    Placenta, 2014;35(12):1049-56.  2014

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