Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.
Store the unopened product at 2° C to 8° C. Do not use past expiration date.
Simultaneously detect the relative levels of these proteases in a single sample.
|Cathepsin A||Kallikrein 6||MMP-12|
|Cathepsin B||Kallikrein 7||MMP-13|
|Cathepsin C/DPPI||Kallikrein 10||Neprilysin/CD10|
|Cathepsin D||Kallikrein 11||Presenilin-1|
|Cathepsin E||Kallikrein 13||Proprotein Convertase 9|
|Cathepsin L||MMP-1||Proteinase 3|
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|Cathepsin B||DCATB0||DY953, DY2176|
|Cathepsin S||DY1183, DY2227|
|Proprotein Convertase 9||DPC900||DY3888|
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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