Proteome Profiler Human Protease Array Kit
Proteome Profiler Human Protease Array Kit Summary
To simultaneously detect the relative changes of 35 proteases in a single sample. No specialized equipment is necessary
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.
Stability and Storage
Store the unopened product at 2° C to 8° C. Do not use past expiration date.
Simultaneously detect the relative levels of these proteases in a single sample.
|Cathepsin A||Kallikrein 6||MMP-12|
|Cathepsin B||Kallikrein 7||MMP-13|
|Cathepsin C/DPPI||Kallikrein 10||Neprilysin/CD10|
|Cathepsin D||Kallikrein 11||Presenilin-1|
|Cathepsin E||Kallikrein 13||Proprotein Convertase 9|
|Cathepsin L||MMP-1||Proteinase 3|
Assays for Analytes represented in the Human Protease Array Kit
|Analyte||Quantikine® ELISA Kits||DuoSet® ELISA Development Systems|
|Cathepsin B||DCATB0||DY953, DY2176|
|Cathepsin S||DY1183, DY2227|
|Proprotein Convertase 9||DPC900||DY3888|
Citations for Proteome Profiler Human Protease Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization
Authors: J Lee, YH Nho, SK Yun, YS Hwang
Integr Cancer Ther, 2019;18(0):1534735419851. 2019
S100A8 & S100A9: Alarmin mediated inflammation in tendinopathy
Authors: LAN Crowe, M McLean, SM Kitson, EG Melchor, K Patommel, HM Cao, JH Reilly, WJ Leach, BP Rooney, SJ Spencer, M Mullen, M Chambers, GAC Murrell, IB McInnes, M Akbar, NL Millar
Sci Rep, 2019;9(1):1463. 2019
Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner
Authors: X Zhang, YS Hwang
Oncol Lett, 2019;18(5):4645-4650. 2019
Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction
Authors: K Saito, M Sakaguchi, S Maruyama, H Iioka, EW Putranto, IW Sumardika, N Tomonobu, T Kawasaki, K Homma, E Kondo
J Cancer, 2018;9(16):2916-2929. 2018
Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3
Authors: SA Greenall, J Donoghue, TG Johns, TE Adams
Transl Oncol, 2018;11(4):971-978. 2018
Intranasal delivery of VEGF enhances compensatory lung growth in mice
Authors: DT Dao, JT Vuong, L Anez-Busti, A Pan, PD Mitchell, GL Fell, MA Baker, DR Bielenberg, M Puder
PLoS ONE, 2018;13(6):e0198700. 2018
Astrocytic tight junctions control inflammatory CNS lesion pathogenesis
Authors: S Horng, A Therattil, S Moyon, A Gordon, K Kim, AT Argaw, Y Hara, JN Mariani, S Sawai, P Flodby, ED Crandall, Z Borok, MV Sofroniew, C Chapouly, GR John
J. Clin. Invest., 2017;0(0):. 2017
HIV Reprograms Human Airway Basal Stem/Progenitor Cells to Acquire a Tissue-Destructive Phenotype
Authors: NPY Chung, X Ou, KMF Khan, J Salit, RJ Kaner, RG Crystal
Cell Rep, 2017;19(6):1091-1100. 2017
Inhibition of cathepsin S confers sensitivity to methyl protodioscin in oral cancer cells via activation of p38 MAPK/JNK signaling pathways
Authors: MJ Hsieh, CW Lin, MK Chen, SY Chien, YS Lo, YC Chuang, YT Hsi, CC Lin, JC Chen, SF Yang
Sci Rep, 2017;7(0):45039. 2017
Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin
Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016
Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells.
Authors: Mohammed F, Khajah M, Yang M, Brackenbury W, Luqmani Y
Int J Oncol, 2015;48(1):73-83. 2015
Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
J Immunol, 2015;195(6):2763-73. 2015
Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
Placenta, 2014;35(12):1049-56. 2014
Shedding of dipeptidyl peptidase 4 is mediated by metalloproteases and up-regulated by hypoxia in human adipocytes and smooth muscle cells.
Authors: Rohrborn D, Eckel J, Sell H
FEBS Lett, 0;588(21):3870-7. 0
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
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