Proteome Profiler Mouse Adipokine Array Kit
Proteome Profiler Mouse Adipokine Array Kit Summary
To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.
- Product Insert
- Troubleshooting Guide
- Assays for analytes represented in the Mouse Adipokine Array Kit
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
| Adiponectin | IGF-I | M-CSF |
| AgRP | IGF-II | OSM |
| ANGPT-L3 | IGFBP-1 | PAI-1/Serpin E1 |
| CCL2/MCP-1/JE | IGFBP-2 | PTX2 |
| CCL5/RANTES | IGFBP-3 | PTX3 |
| CRP | IGFBP-5 | Pref-1 |
| DPPIV/CD26 | IGFBP-6 | RAGE |
| Endocan | IL-6 | RBP4 |
| Fetuin A | IL-10 | Resistin |
| FGF acidic | IL-11 | TIMP-1 |
| FGF-21 | Leptin | TNF-alpha |
| HGF | LIF | VEGF |
| ICAM-1 | Lipocalin-2/NGAL | |
Product Datasheets
Preparation and Storage
Citations for Proteome Profiler Mouse Adipokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Early postnatal soluble FGFR3 therapy prevents the atypical development of obesity in achondroplasia
Authors: C Saint-Laur, S Garcia, V Sarrazy, K Dumas, F Authier, S Sore, A Tran, P Gual, I Gennero, JP Salles, E Gouze
PLoS ONE, 2018;13(4):e0195876. 2018
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Insulin resistance causes inflammation in adipose tissue
Authors: M Shimobayas, V Albert, B Woelnerhan, IC Frei, D Weissenber, AC Meyer-Gers, N Clement, S Moes, M Colombi, JA Meier, MM Swierczyns, P Jenö, C Beglinger, R Peterli, MN Hall
J. Clin. Invest., 2018;0(0):. 2018
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Potential Hypoglycaemic and Antiobesity Effects of Senna italica Leaf Acetone Extract
Authors: RO Malematja, VP Bagla, I Njanje, V Mbazima, KW Poopedi, L Mampuru, MP Mokgotho
Evid Based Complement Alternat Med, 2018;2018(0):5101656. 2018
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Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
Cancer Res, 2017;0(0):. 2017
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Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
Cell. Physiol. Biochem., 2017;44(5):1896-1911. 2017
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Mfn2 is critical for brown adipose tissue thermogenic function
Authors: M Boutant, SS Kulkarni, M Joffraud, J Ratajczak, M Valera-Alb, R Combe, A Zorzano, C Cantó
EMBO J, 2017;0(0):. 2017
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Adipose tissue supports normalization of macrophage and liver lipid handling in obesity reversal
Authors: M Vatarescu, S Bechor, Y Haim, T Pecht, T Tarnovsky, N Slutsky, O Nov, H Shapiro, A Shemesh, A Porgador, N Bashan, A Rudich
J. Endocrinol., 2017;0(0):. 2017
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High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice
Authors: Sneha Sundaram
Anticancer Res, 2016;36(12):6279-6287. 2016
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Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
Authors: Desarzens S, Liao W, Mammi C, Caprio M, Faresse N
PLoS ONE, 2014;9(4):e94127. 2014
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Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
Cytokine, 2013;62(2):206-9. 2013
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Palmitate enhances the differentiation of mouse embryonic stem cells towards white adipocyte lineages.
Authors: Petrighi Polidori G, Lomax M, Docherty K
Mol Cell Endocrinol, 2012;361(1):40-50. 2012
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Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study.
Authors: Sengupta K, Mishra A, Rao M, Sarma K, Krishnaraju A, Trimurtulu G
Lipids Health Dis, 2012;11(0):122. 2012
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Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells.
Authors: Price RS, Cavazos DA, De Angel RE, Hursting SD, Degraffenried LA
Prostate Cancer Prostatic Dis., 2012;15(2):135-43. 2012
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Age and sex mediated changes in epicardial fat adipokines.
Authors: Fei J, Cook C, Blough E, Santanam N
Atherosclerosis, 2010;212(2):488-94. 2010
FAQs
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Tested in 3T3-L1 cells, data matches expectation, very good
