Proteome Profiler Mouse Adipokine Array Kit

Contains 4 membranes - each spotted in duplicate with 38 different obesity-related antibodies
  (13 citations)
(2 Reviews)
    
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Product Details
Citations (13)
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Kit Summary

To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin IGF-I M-CSF
AgRP IGF-II OSM
ANGPT-L3 IGFBP-1 PAI-1/Serpin E1
CCL2/MCP-1/JE IGFBP-2 PTX2
CCL5/RANTES IGFBP-3 PTX3
CRP IGFBP-5 Pref-1
DPPIV/CD26 IGFBP-6 RAGE
Endocan IL-6 RBP4
Fetuin A IL-10 Resistin
FGF acidic IL-11 TIMP-1
FGF-21 Leptin TNF-alpha
HGF LIF VEGF
ICAM-1 Lipocalin-2/NGAL  

Product Datasheets

Certificate of Analysis Lookup

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Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Sample Type
  1. Early postnatal soluble FGFR3 therapy prevents the atypical development of obesity in achondroplasia
    Authors: C Saint-Laur, S Garcia, V Sarrazy, K Dumas, F Authier, S Sore, A Tran, P Gual, I Gennero, JP Salles, E Gouze
    PLoS ONE, 2018;13(4):e0195876.  2018
  2. Insulin resistance causes inflammation in adipose tissue
    Authors: M Shimobayas, V Albert, B Woelnerhan, IC Frei, D Weissenber, AC Meyer-Gers, N Clement, S Moes, M Colombi, JA Meier, MM Swierczyns, P Jenö, C Beglinger, R Peterli, MN Hall
    J. Clin. Invest., 2018;0(0):.  2018
  3. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017;0(0):.  2017
  4. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
    Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
    Cell. Physiol. Biochem., 2017;44(5):1896-1911.  2017
  5. Mfn2 is critical for brown adipose tissue thermogenic function
    Authors: M Boutant, SS Kulkarni, M Joffraud, J Ratajczak, M Valera-Alb, R Combe, A Zorzano, C Cantó
    EMBO J, 2017;0(0):.  2017
  6. Adipose tissue supports normalization of macrophage and liver lipid handling in obesity reversal
    Authors: M Vatarescu, S Bechor, Y Haim, T Pecht, T Tarnovsky, N Slutsky, O Nov, H Shapiro, A Shemesh, A Porgador, N Bashan, A Rudich
    J. Endocrinol., 2017;0(0):.  2017
  7. High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice
    Authors: Sneha Sundaram
    Anticancer Res, 2016;36(12):6279-6287.  2016
  8. Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
    Authors: Desarzens S, Liao W, Mammi C, Caprio M, Faresse N
    PLoS ONE, 2014;9(4):e94127.  2014
  9. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013;62(2):206-9.  2013
  10. Palmitate enhances the differentiation of mouse embryonic stem cells towards white adipocyte lineages.
    Authors: Petrighi Polidori G, Lomax M, Docherty K
    Mol Cell Endocrinol, 2012;361(1):40-50.  2012
  11. Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study.
    Authors: Sengupta K, Mishra A, Rao M, Sarma K, Krishnaraju A, Trimurtulu G
    Lipids Health Dis, 2012;11(0):122.  2012
  12. Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells.
    Authors: Price RS, Cavazos DA, De Angel RE, Hursting SD, Degraffenried LA
    Prostate Cancer Prostatic Dis., 2012;15(2):135-43.  2012
  13. Age and sex mediated changes in epicardial fat adipokines.
    Authors: Fei J, Cook C, Blough E, Santanam N
    Atherosclerosis, 2010;212(2):488-94.  2010
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Average Rating: 5 (Based on 2 reviews)

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  Anonymous 10/19/2017
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: Proteome Profiler Mouse Adipokine Array Kit [ARY013].
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  Anonymous 10/06/2016
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