Proteome Profiler Mouse Adipokine Array Kit
R&D Systems | Catalog # ARY013
Key Product Details
Species
Product Summary for Proteome Profiler Mouse Adipokine Array Kit
Kit Summary
To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
|---|---|---|
| Adiponectin | IGF-I | M-CSF |
| AgRP | IGF-II | OSM |
| ANGPT-L3 | IGFBP-1 | PAI-1/Serpin E1 |
| CCL2/MCP-1/JE | IGFBP-2 | PTX2 |
| CCL5/RANTES | IGFBP-3 | PTX3 |
| CRP | IGFBP-5 | Pref-1 |
| DPPIV/CD26 | IGFBP-6 | RAGE |
| Endocan | IL-6 | RBP4 |
| Fetuin A | IL-10 | Resistin |
| FGF acidic | IL-11 | TIMP-1 |
| FGF-21 | Leptin | TNF-alpha |
| HGF | LIF | VEGF |
| ICAM-1 | Lipocalin-2/NGAL | |
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for Proteome Profiler Mouse Adipokine Array Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Mouse Adipokine Array Kit
For research use only
Related Research Areas
Citations for Proteome Profiler Mouse Adipokine Array Kit
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Customer Reviews for Proteome Profiler Mouse Adipokine Array Kit (11)
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Customer Images
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Verified Customer | Posted 08/15/2023The kit works very well
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Verified Customer | Posted 11/22/2021The serum should be kept in 4 degree fridge or used immediately
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Verified Customer | Posted 02/19/2021Consistent resuts.
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Verified Customer | Posted 02/17/2021Phenomenal kit that is easy to use and reliable.
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Verified Customer | Posted 02/10/2021Outstanding kit.
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Verified Customer | Posted 06/04/2019We evaluated the adipokine profile of two different mice's groups: a high fat diet and a control group fed with a pigment. We observed significant changes in several proteins
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Verified Customer | Posted 04/30/2019
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Verified Customer | Posted 12/11/2018
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Verified Customer | Posted 11/13/2018Tested in 3T3-L1 cells, data matches expectation, very good
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Verified Customer | Posted 10/19/2017
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Verified Customer | Posted 10/06/2016
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