Kit Summary

To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin IGF-I M-CSF
AgRP IGF-II OSM
ANGPT-L3 IGFBP-1 PAI-1/Serpin E1
CCL2/MCP-1/JE IGFBP-2 PTX2
CCL5/RANTES IGFBP-3 PTX3
CRP IGFBP-5 Pref-1
DPPIV/CD26 IGFBP-6 RAGE
Endocan IL-6 RBP4
Fetuin A IL-10 Resistin
FGF acidic IL-11 TIMP-1
FGF-21 Leptin TNF-alpha
HGF LIF VEGF
ICAM-1 Lipocalin-2/NGAL  
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Sample Type
  1. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017;0(0):. 2017
  2. Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
    Authors: Desarzens S, Liao W, Mammi C, Caprio M, Faresse N
    PLoS ONE, 2014;9(4):e94127. 2014
  3. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013;62(2):206-9. 2013
  4. Palmitate enhances the differentiation of mouse embryonic stem cells towards white adipocyte lineages.
    Authors: Petrighi Polidori G, Lomax M, Docherty K
    Mol Cell Endocrinol, 2012;361(1):40-50. 2012
  5. Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study.
    Authors: Sengupta K, Mishra A, Rao M, Sarma K, Krishnaraju A, Trimurtulu G
    Lipids Health Dis, 2012;11(0):122. 2012
  6. Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells.
    Authors: Price RS, Cavazos DA, De Angel RE, Hursting SD, Degraffenried LA
    Prostate Cancer Prostatic Dis., 2012;15(2):135-43. 2012
  7. Age and sex mediated changes in epicardial fat adipokines.
    Authors: Fei J, Cook C, Blough E, Santanam N
    Atherosclerosis, 2010;212(2):488-94. 2010
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Average Rating: 5 (Based on 2 reviews)

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