Protocadherin-1 Antibody (5D5) - Azide and BSA Free

Novus Biologicals | Catalog # H00005097-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5D5

Format

Azide and BSA Free
View all Protocadherin-1 Primary Antibodies »

Product Specifications

Immunogen

PCDH1 (NP_002578 62 a.a. - 169 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFA

Localization

Cell junction. Cell membrane. Single-pass type I membrane protein. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries.

Specificity

PCDH1 - protocadherin 1 (cadherin-like 1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Protocadherin-1 Antibody (5D5) - Azide and BSA Free

Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01] - PCDH1 monoclonal antibody (M01), clone 5D5 Analysis of PCDH1 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Immunocytochemistry/Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01]

Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01]

ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01]

ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Detection limit for recombinant GST tagged PCDH1 is approximately 0.03ng/ml as a capture antibody.

Applications for Protocadherin-1 Antibody (5D5) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protocadherin-1

This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

Alternate Names

PC42, PCDH1, PCDH42, Protocadherin1

Entrez Gene IDs

5097 (Human)

Gene Symbol

PCDH1

OMIM

603626 (Human)

UniProt

NP_002578 (Human)

Additional Protocadherin-1 Products

Product Documents for Protocadherin-1 Antibody (5D5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Protocadherin-1 Antibody (5D5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cadherin Superfamily

Citations for Protocadherin-1 Antibody (5D5) - Azide and BSA Free

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Protocols

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FAQs

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