PRRX1 Antibody (CL14471) - BSA Free

Novus Biologicals | Catalog # NBP3-26856

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # CL14471

Format

BSA Free
View all PRRX1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P54821, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for PRRX1 Antibody (CL14471) - BSA Free

PRRX1 Antibody (CL14471)

Immunohistochemistry-Paraffin: PRRX1 Antibody (CL14471) [NBP3-26856]

Analysis in human cervix, uterine and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
PRRX1 Antibody (CL14471)

Immunohistochemistry-Paraffin: PRRX1 Antibody (CL14471) [NBP3-26856]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.
PRRX1 Antibody (CL14471)

Immunohistochemistry-Paraffin: PRRX1 Antibody (CL14471) [NBP3-26856]

Staining of human endometrium shows strong nuclear positivity in glandular cells.
PRRX1 Antibody (CL14471)

Immunohistochemistry-Paraffin: PRRX1 Antibody (CL14471) [NBP3-26856]

Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.
PRRX1 Antibody (CL14471)

Immunocytochemistry/ Immunofluorescence: PRRX1 Antibody (CL14471) [NBP3-26856]

Staining in U-138MG cell line showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
PRRX1 Antibody (CL14471)

Immunohistochemistry-Paraffin: PRRX1 Antibody (CL14471) [NBP3-26856]

Staining of human prostate shows strong nuclear positivity in glandular cells.

Applications for PRRX1 Antibody (CL14471) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRRX1

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns.

Alternate Names

Homeobox protein PHOX1, paired mesoderm homeo box 1, paired mesoderm homeobox 1 isoform pmx-1b, paired mesoderm homeobox protein 1, paired related homeobox 1, Paired-related homeobox protein 1, PHOX1PRX-1, PMX1PRX1

Gene Symbol

PRRX1

Additional PRRX1 Products

Product Documents for PRRX1 Antibody (CL14471) - BSA Free

Certificate of Analysis

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Product Specific Notices for PRRX1 Antibody (CL14471) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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