PRRX1 Antibody

Immunocytochemistry/ Immunofluorescence: PRRX1 Antibody [NBP1-06067] -

Immunocytochemistry/ Immunofluorescence: PRRX1 Antibody [NBP1-06067] - PRRX1 & PRRX2 bind to mir875 promoter region. (a) Diagram of mir875 promoter region. The CAGE peak accurately represents TSS. (b) Immunohistochemistry image showing PRRX1 (green) & DAPI staining of nuclei (blue) in E14 tooth germ. (c) qRT-PCR analysis of Prrx1 & Prrx2 expressions in teeth obtained from E13 to P7 after normalization to Gapdh expression. (d) Schematic diagram showing luciferase reporter vector of mir875 promoter. PRRX1/2 binding was shown to occur in the mir875 promoter region. TAATTA is a potential binding site for PRRX1/2. (e) Mir875 promoter reporter activities were evaluated in mDP cells transfected with a Prrx1/2 expression vector after 12, 24, & 48 hours of culture. (f) qRT-PCR analysis of miR875-5p expression in mDP cells cultured with or without Prrx1/2 transfection. (g) Sequences of mir875 promoter & mutant promoter. TAATTA was replaced by the TCCCCA sequence. (h) Mir875 promoter reporter activities were evaluated in mDP cells after transfection with a Prrx1 or Prrx2 expression vector. *P < 0.05. Error bars represent the mean ± S.D. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32188878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.